MABBI – Research conducted by Irandi Putra Pratomo, Yumiko Koba, Eiso Hiyama, Wisnu Ananta Kusuma, Rafika Indah Paramita, Linda Erlina, Fadilah Fadilah and Aryo Tedjo from Indonesia University, Jakarta, Indonesia; Hiroshima University, Hiroshima, Japan; IPB University, Bogor, Indonesia entitled In-silico cross-validation unveiled genomic mortality signatures of CC5 and CC8 MRSA obtained from the intensive care patients.
The clonal complex (CC) 5 and 8 methicillin-resistant Staphylococcus aureus (MRSA) are among the deadliest pathogens in the intensive care unit (ICU) setting. In-silico next-generation sequencing data analysis allows vast and rapid detection of microbial virulence signatures. This study aimed to identify the virulence signatures of clinical CC5 and CC8 MRSA and analyze its correlation with the mortality of ICU patients treated at a teaching hospital in Japan between 2013 and 2014. Fifty-two MRSA genomes, obtained from the specimen of the patients, were assembled using an Illumina MiSeq (Illumina Inc., San Diego, CA) platform. The MRSA genomic signatures were annotated using the annotation tools for housekeeping and virulence genotyping (Center for Genomic Epidemiology DTU, Lyngby, Denmark). Orange Data Mining (University of Ljubljana, Ljubljana, Slovenia), applying its confusion matrix for neural network cross-validation method, was used to infer the correlation of genomic signature with patient mortality. This study revealed that the patient mortality caused by CC5 and CC8 MRSA was 15.15% (5/33) and 42.10% (8/19), respectively. The detection of ΔclfA and Δsel, and the bacterial adherence pattern of clfA-eap were found in CC5 MRSA deaths. The detection of capJ+, sdrC+, ΔclfA, clfA−, and Δsel, and the bacterial adherence pattern of clfA-eap and eap-sdrC were found in CC8 MRSA deaths.This in-silico study found genomic signatures related to the bacterial adherence properties to predict patient mortality between different MRSA CCs. The CC8 MRSA was shown to be more virulent and lethal compared to the CC5 MRSA. (Tri/MABBI)
Read more: https://doi.org/10.1111/resp.14149_16
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