Metabolite profiling of Andrographis paniculata leaves and stem extract using UHPLC-Orbitrap-MS/MS

MABBI – Research conducted by Mohamad Rafi, Alfi Hudatul Karomah, Rudi Heryanto, Dewi Anggraini Septaningsih, Vishnu Ananta Kusuma, Muhammad Bachri Amran, Abdul Rohman and Bambang Prajogo from IPB University, Airlangga University, Gadjah Mada University and Bandung Institute of Technology entitled Metabolite profiling of Andrographis paniculata leaves and stem extract using UHPLC-Orbitrap-MS/MS
Andrographis paniculata is known as the king of bitters and it has been widely used as a medicinal plant. The properties of A. paniculata are generally determined by the metabolite composition, which may be influenced by several factors, one of which is the part of the plant extracted. The objectives of this research are to identify putatively the metabolite composition of the stem and the leaf extracts using LC-MS/MS and classify them using PCA. The stem and the leaves samples were separated and extracted using sonication with 70% ethanol. A total of 31 metabolite compounds has been putatively identified. All compounds were identified in the stem and the leaves extracts, which only differed in their intensity. These metabolite compounds were divided into diterpene lactones, flavonoids, and phenolic acid groups. By using the peak intensities of the 18 compounds identified, the leaves and stem extracts were grouped using PCA. (Tri/MABBI)



Read more: 

https://www.tandfonline.com/doi/abs/10.1080/14786419.2020.1789637

Comments

Leave a Reply

Your email address will not be published. Required fields are marked *