MABBI – Research conducted by Badrut Tamam, Dahrul Syah, Maggy Thenawidjaja Suhartono, Vishnu Ananta Kusuma, Shinjiro Tachibana, and Hanifah Nuryani Lioe from Bogor Agricultural University, University of the Ryukyus and Polytechnic of Health Bali entitled Proteomic study of bioactive peptides from tempe
The country. Studies on the bioactive peptides in tempeh are rare. Here, we studied bioactive peptides in samples from three tempe producers with different degrees of sanitation. The peptide sub-fractions of tempeh from each producer were collected following water extraction, ultrafiltration (<3 kDa), gel filtration chromatography, and reversed phase high performance liquid chromatography (RP-HPLC) separation followed by LC-MS. The MS spectra were then predicted using FindPept tools (https://web.expasy.org/findpept/), and their biofunctionalities were confirmed with BIOPEP databases (www.uwm.edu.pl/biochemia/index.php/pl/biopep ). There were several similar peptides found in tempeh from the three producers. Peptides Val-His and Ala-Leu-Glu-Pro were found in tempeh from all producers. Producers having a good sanitation level had more bioactive peptides than those with moderate or poor sanitation levels (58%, 43% and 35%, from good to poor sanitation). This work showed that the tempe from the three producers had antihypertensive, antidiabetic, antioxidative and antitumor peptides. (Tri/MABBI)
Read more: https://pubmed.ncbi.nlm.nih.gov/30930003/
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