MABBI – Research conducted by Mohamad Rafi, Laela Wulansari, Dewi A Septaningsih, Tsania F Purnomo, Reza Auliatifani, Auliya Ilmiawati, Wina Yulianti, Nunuk K Nengsih, Irma H Suparto, and Wisnu A Kusuma from IPB University entitled Antioxidant Capacity and Phytochemical Profile of Punica granatum L. Peel Extracts Using Different Solvent Extraction.
Pomegranate is known to have valuable nutrients and contains various bioactive compounds found in the fruit’s peel. The utilization of these bioactive compounds could be used as herbal medicines and supplements, such as antioxidants. This study aimed to determine the antioxidant capacity, phytochemical profile, and pomegranate peel extract grouping using different extracting solvents. The extraction solvents used were water, 70% ethanol, and ethanol pro analysis (p.a) grade. The antioxidant capacity of the three extracts was measured using DPPH and CUPRAC methods. We also determined the total phenolic and flavonoid levels and the TLC fingerprint analysis and FTIR spectrum of the pomegranate peel extracts. The 70% ethanol extract owned the largest antioxidant capacity. It was significantly different from the other two extracts with a value of 358.67 and 2981.59 µmol trolox/g dried sample using the DPPH and CUPRAC methods, respectively. The three pomegranate peel extracts’ total phenolic and flavonoid levels ranged from 287.26–1068.81 mg GAE/g dried sample and 0.24-0.75 mg QE/g dried sample. TLC fingerprint analysis of pomegranate peel extract yielded 2, 6, and 6 bands for water extract, 70% ethanol, and p.a ethanol, respectively. The three extracts can be grouped based on FTIR spectrum data using principal component analysis using three principal components with a total variance of 93%. The results obtained showed that using different extracting solvents provides different antioxidant capacities and phytochemical profiles (Tri/MABBI).
Read more: https://doi.org/10.11594/jtls.11.03.14
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